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ValGenetics analyzes CYVCV in citrus using molecular techniques

The Valencian biotechnology company has added a conventional RT‑PCR and a real‑time RT‑PCR to its services for the detection of the emerging Citrus Yellow Vein Clearing Virus (CYVCV), providing its clients with a sensitive, fast and reliable diagnostic method.

By Redacción ECA

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The Citrus yellow vein clearing virus (CYVCV) is an emerging virus affecting citrus crops and has been included on the alert list of the European and Mediterranean Plant Protection Organization (EPPO), reflecting its phytosanitary relevance.

In the context of citrus cultivation—of great strategic importance for the Mediterranean region—CYVCV has gained prominence following its detection in Spain in 2025. It is a pathogen capable of infecting most citrus species, although it is particularly significant in lemon due to its higher susceptibility.

Among the most characteristic symptoms associated with this disease are yellowing of leaf veins, leaf deformation and chlorotic ring spots. In some cases, vein necrosis and fruit malformations may also appear, directly affecting commercial quality. In severe infections, lemon trees may show general weakening and a marked reduction in yield.

The virus can be transmitted through grafting, contaminated tools and various vectors, including the aphids Aphis spiraecola, Aphis aurantii, Aphis craccivora and Aphis gossypii, as well as the citrus whitefly Dialeurodes citri.

Photo ValGenetics

Given this expansion scenario and considering that no effective curative treatments currently exist for infected plants, management strategies rely mainly on prevention. The use of certified plant material, vector control and proper tool disinfection are essential measures to minimize virus spread. In this context, early detection plays a key role in containing its dissemination and reducing the economic impact on the citrus sector.

To address this need, the biotechnology company ValGenetics has implemented two advanced molecular diagnostic techniques: a conventional RT‑PCR and a real‑time RT‑PCR, both designed to provide specific, sensitive and rapid identification of CYVCV. These tools enhance phytosanitary surveillance capacity and support decision‑making in nurseries, laboratories and agricultural operations.

In this regard, Dr. Ana Crespo, Head of Phytopathology at ValGenetics, highlights that one of the sector’s main needs is to strengthen diagnostics and phytosanitary monitoring. “Right now, the priority is to support nurseries and the entire sector with studies and tools that enable rapid action,” she explains.

According to Dr. Crespo, it is essential to have diagnostic systems that streamline the screening of large volumes of plant material, especially in nurseries and certification programs, allowing early detection of potentially infected plants and helping to contain the virus.

With these new diagnostic tools, ValGenetics reinforces its commitment to innovation and plant health, offering the citrus sector solutions that improve early detection of CYVCV and contribute to its containment.

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